Developmental timing and RNA-mediated gene regulation

Pepper, AS-R, McCane J, Kemper K, Au Yeung D, Lee R, Ambros V, Moss EG.
The C. elegans heterochronic gene lin-46 affects developmental timing at two larval stages
 and encodes a relative of the scaffolding protein gephyrin.
Development 2004 Apr. 8; 131(9):2049-59. pdf

Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E and Ambros V.
Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation.
Genome Biology 2004 Feb. 16; 5:R13. pdf

Moss EG and Taylor JM.
Small interfering RNAs in the radar of the interferon system.
Nature Cell Biology 2003 Sept. 1; 5:771-772. News and Views . pdf

Yang D-H and Moss EG.
Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse.
Gene Expr Patterns. 2003 Dec;3(6):719-26. pdf

Bellacosa A and Moss EG.
RNA Repair: Damage Control.
Curr Biol. 2003 June 17;13(12):R482-R484. Review. pdf

Moss EG and Tang L.
Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites.
Dev Biol. 2003 June 15;258(2):432-42. pdf

Moss EG
Silencing Unhealthy Alleles Naturally
Trends Biotech. 2003 May; 21(5):185-187. Review. pdf

Moss EG
"MicroRNAs"
In Noncoding RNAs: Molecular Biology and Molecular Medicine
J. Barciszewski, V.A. Erdmann, eds. Landes Bioscience. 2003. pdf

Moss EG and Poethig RS.
MicroRNAs: something new under the sun.
Curr Biol. 2002 Oct 15;12(20):R688-90. Review. pdf

Seggerson K, Tang L, Moss EG. 
Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation.
Dev Biol. 2002 Mar 15;243(2):215-25. pdf

Moss EG. 
MicroRNAs: hidden in the genome.
Curr Biol. 2002 Feb 19;12(4):R138-40. Review. pdf

Moss EG. 
RNA interference: it's a small RNA world.
Curr Biol. 2001 Oct 2;11(19):R772-5. Review. pdf

Moss EG. 
Non-coding RNA's: lightning strikes twice.
Curr Biol. 2000 Jun 15;10(12):R436-9. Review. pdf

Moss EG, Lee RC, Ambros V. 
The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA.
Cell. 1997 Mar 7;88(5):637-46. pdf
 
Protocols:
Polysome prep I
Polysome prep II
Sucrose gradients
Buffer U
Large-scale synchronized worm preps
unc-119 co-bombardment using liquid culture

C. elegans online - annotated links to worm-related sites

a predicted microRNA-target duplex

Lin28 expression along the crypt/villus axis

homology model of LIN-28 protein structure