Lab 8 Peripheral Nervous System: 1) Index 2) Introduction 3) Things To identify 4) Nerve and Nerve Fibers 5) Nerve & Nerve Fibers II 6) Nerve & Nerve Fibers III 7) Dorsal Root Ganglion 8) Sympathetic Ganglion 9) Parasympathetic Ganglion 10)Practical

 

SYMPATHETIC GANGLION

 

# 66 - Sympathetic ganglion, H&E, human, 1.5 mm

# 222 - Sympathetic ganglion, l.s., H&E

# 67 - Sympathetic ganglion, silver (this slide has been discontinued, so you may not have it)

 

Slide 66 is an excellent preparation that will allow you to compare the histology of connective tissue (collagen) fibers, smooth muscle and nerve fibers found in this section. Be certain that you are able to distinguish these tissues. This section shows several groups of neurons distributed throughout the ganglion. The ganglion cells can be identified even at low magnification by their prominent nuclei and nucleoli. These multipolar neurons are slightly smaller that those of the dorsal root ganglion. Their nuclei are eccentrically placed and their cytoplasm is basophilic. Nissl substance may be distributed near the nucleus or at the periphery of the cytoplasm. The neurons are surrounded by satellite cells. You may detect yellow-brown lipofuchsin granules in the cytoplasm of the ganglion cells.

Slide 222 shows the sympathelic ganglion in longitudinal section, whereas slide 67 is a silver preparation that demonstrates nerve fibers but which is not good for cellular detail.

References: Ross, p. 270, Fig. 11.15 Gartner, p. 141, Fig.1 - 2 Wheater, p. 129, Fig. 7.18 DiFiore, p. 97, Fig. 7-16

 

This is a slide from the Sympathetic Ganglion. The blue arrows are pointing to the eccentric placed nuclei , which is the key to differentiate between the sympathetic ganglion and the Dorsal Root Ganlion. These Neurons are large for the same reason as mentioned earlier, but these are Multipolar Motor (efferent) Neurons, responsible for conveying information from the Central Nervous System.

THe red arrows are showing nuclei of the satellite cells. The green arrow is showing an example of Lipofushkin.

Blue arrow - Eccentric Place Nucli of Motor Nuerons

Green arrow - Lipofushckin

Red arrow - Satellite cells

Sympathetic Ganglion (Multipolar Neurons)