| LAB 6 Blood and Myeloid Tissue: 1) Introduction 2) Erythrocytes and Platelets 3) Neutrophils 4) Basophils 5) Monocytes 6) Eosinophils 7) Bone Marrow Smear 8)Practical |
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# 71 - Bone Marrow Smear, Wright Stain
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The study of myeloid or hematopoietic tissue is beyond the scope of this course and will be treated extensively in Hematology next year. You are required to know the structure and function of the megakaryocyte. The megakaryocyte fragments off parts of its cytoplasm to form the platelets that circulate in peripheral blood. Examine this slide and Figure 9.14 in Ross to identify megakaryocytes. Examine their size in relation to other cells found in the marrow cavity on this slide. Remember that the metakaryocyte has a single, multilobed nucleus which can serve to distinguish it from osteoclasts which are large, multinucleated cells at the periphery of the spicules of the bone. Review the development of erythrocytes. Relate the histological changes that take place as the cells proceed from the basophilic normoblasts to mature erythrocytes. References: Gartner, pp. 88 - 93 Ross, pp. 208 DiFiore, pp. 68 - 73 Wheater, Chapter 3, pp. 54 -59 |
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This is a slide of a Bone Marrow smear. The white arrow is pointing the the huge Megakaryocyte. For comparison sake the yellow arrow is pointing to a Red Blood Cell. No cell looks like the megakaryocyte compared to it's surrounding cells, and it is easy to remember (MEGA is HUGE). They are resposible for the synthesis of platelets. Platelets are made by budding off the megakaryocytes membrane. White arrow - Megakaryocyte Yellow arrow - Red Blood Cell |
Bone Marrow Smear |
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