Lab 2 Epithelial Tissue: Index - 1)Introduction - 2)Simple Squamous - 3)Simple Cuboidal - 4)Simple Columnar - 5)PsuedoStratified - 6)Transitional - 7)Stratified Squamous - 8)Stratified Columnar - 9)Stratified Cuboidal - 10) Trachea and Esophogus Comparison - 12) Practical

 

SIMPLE COLUMNAR EPITHELIUM

Tall prismatic cells, with basal nuclei, all at the same level, usually have either an absorptive or secretory function; found lining much of the digestive tract and larger ducts of glands.

 

1. lining of digestive tract

a. # 133 - Gall bladder, monkey, h&e

 

See p. 526 - 527 in Ross for a low power overview of gall bladder histology. Then microscopically observe the tall columnar cells that cover the luminal surface of the gall bladder for a good example of simple columnar epithelium.

References: Ross, p. 526 - 527, Fig. 1 - 4 Gardner, p. 309, Fig. 3 - 4 Wheater. p. 78, Fig. 5.3 a,b

 

The yellow arrow is pointing to a single columnar cell lining the gall bladder. Notice how the nuclei are virtually in the same plane, exemplified by the green line.

Green line- Nuclei of epithelial cells

Yellow Arrow - Single columnar epithelial cell

Epithelium of Gall Bladder

 

 

b. # 112 - Stomach, Fundus, monkey, h&e

If needed #214 - Stomach, combination

 

Note the tall surface mucous cells that line the stomach lumen. These are not goblet cells.

References: Gartner, p. 283, p. 285, Fig. 1, Fig. 3 Fig. 3 Ross, p. 78, Fig. 4.26 p. 264, Fig. 1 p. 86, Fig. 3 Wheater, p. 253, Fig. 14.7c

 

c. # 115 - Duodenum, monkey, h&e

If needed, # 215 - Duodenum, jejunum, ileum

 

The epithelium of the intestinal mucosa is of simple columnar epithelium, but differs from the stomach in that more than one cell type is present. Thus, in addition to the tall columnar cells, look for the occasional mucus-containing goblet cell.

References: Gartner , p. 31, Fig. 3 p. 288, Fig. 1b, Fig. 3a Ross - p. 78, Fig. 4.25 p. 267, Fig. 1b, Fig. 3a p. 485, Fig. 2 DiFiore, p. 13, Fig. 1-4 Wheater, p. 263, Fig. 14.19

 

Again here notice the nuclei of the simple columnar cells lining the duodenum are all in the same plane indicated by the green line

The orange arrow is pointing to a goblet cell, which line the intestinal mucosa. You can see the mucus portion of it (light colored part), that will be secreted into the lumen

Can you notice the lightly stained line that is bordering the superior borders of all the cells (the purple arrows). This is the brush border. This is really microvilli, that are used for absorption in the small intestine.

Epithelium of Duodenum

Green Line - nuclei of epithelial cells

Orange Arrow - Goblet Cell

purple arrows - Brush border

 

d. # 117, 121 or 122 - Ileum, h&e

If needed # 215 - Duodenum, jejunum and ileum, h&e

 

Note the increasing number of goblet cells interspersed among the tall intestinal lining cells.

References: Gartner, p. 289, Fig. 4

 

This is a high power shot of the intestinal lining of the Ileum. Here you can even make out the microvilli that the green arrow is pointing to. These are what make up the brush border.

You also get a nice view of a goblet cell that the blue arrow is pointing to.

Blue Arrow - Goblet cell

Green Arrow - Brush border (microvilli)

Epithelium of Ileum

 

2. lining of oviducts

# 227 - Fallopian tube, h&e

If needed, see # 179 - Fallopian tube, fimbriae, human h&e

 

The lining of the oviduct consists of columnar epithelial cells some of which are ciliated.

References: Gartner, p. 351, Figs. 1 - 2 Ross, p. 694, Fig. 22.16 p. 720, Fig. 2 Wheater, p. 79, Fig. 5.4 (a, b)

 

Again another example of simple columnar cells with in the oviduct. Can you tell if these are cilia or microvilli? Ask yourself can you see spaces between them? Here you can so the blue arrows are pointing to cilia.

Blue Arrow - Cilia

Green arrow and bracket - Ciliated cell

Epithelium of Oviduct

 

3. papillary ducts of kidney

# 142 - Kidney, adrenal, human fetus

# 5 - Simple columnar epithelium, human, kidney

 

Examine the section by eye and then by 4x. In the renal medulla follow the collecting ducts which you located previously (lined with simple cuboidal epithelium) until several unite to form large papillary ducts which are lined with classical simple columnar epithelium. Look for the presence of these linear ducts near the base of the papillae.

References: Gartner, p. 331, Fig. 3Wheater, p. 302, Fig. 16.22 renal papilla Di Fiore, p. 253, Fig. 16-5

 

This is a great example simple columnar epithelium. The green arrow is pointing one of them out. Notice how the cells are taller than they are wide and the nuclei are again all in the same plane. Is there a brush border present? No.

Green Arrow - Simple Columnar epithelial cell

Epithelium of Papillary Duct

 

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