| Lab 19 Esophagus and Stomache: 1) Index 2) Introduction 3) Things to Identify 4) Upper Esophagus 5) Middle Esophagus 6) Lower Esophagus 7) Cardiac Stomach 8) Fundic Stomach 9) Pyloric Stomach 10) Stomach-Duodenal Junction 11) Comparison of Structures 12) Practical |
|
Fundic
Stomach
|
|
# 112 - Stomach, fundus, h - e, monkey # 214 - Stomach, combination, h-e |
|
Identify all the layers of the wall of the stomach. Identify the gastric pits and the numerous fundic glands which extend from these pits to the muscularis mucosae. The lumen of these glands is so narrow as to suggest a cord-like arrangement of cells. Compare the amount of lamina propria found between these glands to that found between the cardiac and pyloric glands. In the fundic region, the pits are narrow and straight with long tubular glands opening into their base. The two major populations of cells in the fundic region are the chief cells (producing zymogens or proenzymes) which are slightly basophilic in h-e preps because of their rER content, and the parietal or oxyntic cells (hydrogen chloride-producing) which appear as large, eosinophilic cells studded along the length of the glands. These cells have the appearance of pink "fried eggs", often are binucleate and characterize the fundic stomach. Near the opening of the glands into the gastic pits are pale-staining mucous neck cells. A fourth cell type, the argentaffin or enteroendocrine cell, is present but requires special staining for visualization. Examine your H & E stained slide to find a clear - staining cell with its nucleus close to the lumen and its cytoplasm directed towards the connective tissue. Shaped like a Hershey kiss, its broad base rests on the basement membrane, Its hormone-like secretions can easily diffuse into capillaries in the underlying lamina propria. You may be able to distinguish more than one layer in the muscularis mucosae. (The muscularis externa is trilaminar at this level, but it is difficult to distinguish more than two layers.) Identify the ganglion cells belonging to the myenteric plexus of Auerbach. REFERENCES:Gartner, p. 283, Fig. 2 - 4 p. 285, Fig. 1 - 2Ross, p. 447, 476 - 483 p. 265, Fig. 1 - 2 DiFiore, p. 183 - 187, Fig. 12-6 - 12-8 Wheater, p. 253 - 255, Fig. 14.7 - 14.9 |
|
This is an image of the Fundic Stomach. The red arrow is showing the large Mucosa, the green arrow is pointing to the Submucosa. Note the prominent Muscularis Mucosa. The fundic stomach also has Gastric pits indicated by the black arrows, but they are not that deep. The major identifiable characteristic of the fundic stomach are the long Fundic Glands, which stretch from the muscularis mucosa to the lumen, which are indicated by the blue arrows. They are not very obvious but if you look carefully you can make out these parallel cords which are the glands. When ever you see these along with gastric pits, you know it is the fundic stomach. Red arrow - Mucosa Black arrow - Gastric Pits White arrow - Muscularis Mucosa Green arrow - Submucosa Blue arrow - Gastric Glands
Here is another look at the make up of the mucosa of the fundic stomach. The red arrow is indicating the direction of the Parallel cords of the fundic glands. They are very suttle, can you make them out? Red arrow - Parallel cords of Fundic Glands
This is a high power shot of the Fundic Glands to see the cells that make them up. The green arrows are showing the lumens of the fundic glands. There are two major cell types that can be found here. The first type are the Parietal or Oxyntic Cells, indicated by the red arrows. These are very easy to identify because they look like "fried eggs", can you see the resemblance? Once you know they are parietal cells, all the cells that don't look like that are the other type of cell which are Chief Cells, indicated by the blue arrows.
Green arrow - lumens of the fundic glands Blue arrow - Chief Cells Red arrow - Parietal Cells
If you want to see an Auerobach's Plexus, refer back to Lab 8 Peripheral Nervous System |
Fundic Stomach
Fundic Glands
Parietal & Chief Cells |
